Promising role of Metformin in reducing the viability of breast cancerous cells

To evaluate the anticancerous effects of different dilutions of metformin were evaluated for in vitro anti-cancerous effects, primarily breast cancer cells (MCF-7, MDA-MB-231). This prospective experimental study was conducted in Department of Pharmacology & Therapeutics BMSI in alliance with PCMD. The duration of study was from March 2016 to February 2017. For evaluating the anticancerous effects of different dilutions of Metformin (0.5µM -100µM) we used 4 different cancerous cells lines; MCF-7, HT-29, MDA-MB-231 and Hela. For assessment of anticancerous effects we used MTT assay by which assessed IC50, SI, % viability of all cells and Trypan blue exclusion assay for only MCF-7 cell line. The % viability of MCF-7 was significantly decreases (?2 (2) = 26.48, p=<0.001) in dose dependent manner from 99.8±0.2 to 39.71±1.3. For MDA-MB-231% viability significantly reduced (?2 (2) =26.48, p=<0.001) from 99.474± 0.298 to 51.55±4. However Metformin had statistically no significant dose dependent effects on % viability of MCF-10 (?2 (2) = 11.709, p=0.069). Metformin significantly exhibited the anticancerous effects on breast cancerous cells by selectively target the cancerous cells without any effects on normal epithelial cells of breast.