Effect of 25(OH)D supplementation on adipokines and advanced glycation end products in experimental type 2 diabetic and obesity rat model

Diabetes mellitus is a global health problem and vitamin D deficiency is thougt to be a reasonal factor for development of diabetes. Our aim is to investigate among vitamin D levels and blood glucose, insulin resistance, adiponectin, apelin, tumor necrosis factor-alpha (TNF-?), interleukin-6 (IL-6), oxidative stress index (OSI) levels and advanced glycation end products (AGEs) formation in modeled obesity and type 2 diabetes mellitus (T2DM) rats. During the 14-week experiment; Control group was fed standart diet-(12 % of calories of fat), Diabetes group was fed high fat diet (HFD)-at the 4th week Streptozotocin (STZ) (35 mg/kg) was injected, Diabetes/vitamin D group was fed HFD-at the 4th week STZ was injected-between 4th-14th week vitamin D supplement was administered, Obesity group was fed HFD-(40% of calories as fat), Obesity/vitamin D group was fed HFD-between 4th-14th week vitamin D supplement was administered. At 14th week, biochemical parameters, adipokine concentrations, kidney tissue cytokine levels, liver tissue oxidative stress index parameters were measured by using ELISA method and Hematoxylin?Eosin staining of kidney, heart, liver, coronary/renal artery tissues and immunohistochemical staining of kidney tissues were performed. Vitamin D can alleviate glucose and lipid parameters and we think vitamin D may be useful adjuvant agent in obesity and T2DM treatment.